ecm1 proteintech 11521 1 ap Search Results


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Proteintech rabbit anti ecm1 antibody
Expression of <t> ECM1 </t> in HCC and normal liver tissues
Rabbit Anti Ecm1 Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Expression of  ECM1  in HCC and normal liver tissues

Journal: World Journal of Surgical Oncology

Article Title: ECM1 promotes migration and invasion of hepatocellular carcinoma by inducing epithelial-mesenchymal transition

doi: 10.1186/s12957-016-0952-z

Figure Lengend Snippet: Expression of ECM1 in HCC and normal liver tissues

Article Snippet: The sections were then immunostained with primary antibody of rabbit anti-ECM1 antibody (11521-1-AP, Proteintech Group, USA) and mouse anti-Vimentin antibody (ZM-0260, ZBGB-BIO) at 4 °C overnight.

Techniques: Expressing

Correlation between the expression of  ECM1  in 120 HCC tissues and their clinicopathological characteristics

Journal: World Journal of Surgical Oncology

Article Title: ECM1 promotes migration and invasion of hepatocellular carcinoma by inducing epithelial-mesenchymal transition

doi: 10.1186/s12957-016-0952-z

Figure Lengend Snippet: Correlation between the expression of ECM1 in 120 HCC tissues and their clinicopathological characteristics

Article Snippet: The sections were then immunostained with primary antibody of rabbit anti-ECM1 antibody (11521-1-AP, Proteintech Group, USA) and mouse anti-Vimentin antibody (ZM-0260, ZBGB-BIO) at 4 °C overnight.

Techniques: Expressing

The correlation between  ECM1  and Vimentin expression in HCC tissues

Journal: World Journal of Surgical Oncology

Article Title: ECM1 promotes migration and invasion of hepatocellular carcinoma by inducing epithelial-mesenchymal transition

doi: 10.1186/s12957-016-0952-z

Figure Lengend Snippet: The correlation between ECM1 and Vimentin expression in HCC tissues

Article Snippet: The sections were then immunostained with primary antibody of rabbit anti-ECM1 antibody (11521-1-AP, Proteintech Group, USA) and mouse anti-Vimentin antibody (ZM-0260, ZBGB-BIO) at 4 °C overnight.

Techniques: Expressing

a Kaplan-Meier analysis of overall survival curve of HCC patients according to ECM1 expression. The HCC patients with ECM1-positive showed significantly shorter overall survival survival rates than those with ECM1-negative. b Kaplan-Meier analysis of disease-free survival curve of HCC patients according to ECM1 expression. The expression of ECM1 is associated with decreased disease-free survival rate

Journal: World Journal of Surgical Oncology

Article Title: ECM1 promotes migration and invasion of hepatocellular carcinoma by inducing epithelial-mesenchymal transition

doi: 10.1186/s12957-016-0952-z

Figure Lengend Snippet: a Kaplan-Meier analysis of overall survival curve of HCC patients according to ECM1 expression. The HCC patients with ECM1-positive showed significantly shorter overall survival survival rates than those with ECM1-negative. b Kaplan-Meier analysis of disease-free survival curve of HCC patients according to ECM1 expression. The expression of ECM1 is associated with decreased disease-free survival rate

Article Snippet: The sections were then immunostained with primary antibody of rabbit anti-ECM1 antibody (11521-1-AP, Proteintech Group, USA) and mouse anti-Vimentin antibody (ZM-0260, ZBGB-BIO) at 4 °C overnight.

Techniques: Expressing

Univariate survival analysis of OS and DFS in 120 patients with HCC

Journal: World Journal of Surgical Oncology

Article Title: ECM1 promotes migration and invasion of hepatocellular carcinoma by inducing epithelial-mesenchymal transition

doi: 10.1186/s12957-016-0952-z

Figure Lengend Snippet: Univariate survival analysis of OS and DFS in 120 patients with HCC

Article Snippet: The sections were then immunostained with primary antibody of rabbit anti-ECM1 antibody (11521-1-AP, Proteintech Group, USA) and mouse anti-Vimentin antibody (ZM-0260, ZBGB-BIO) at 4 °C overnight.

Techniques:

Multivariate survival analysis of OS and DFS in 120 patients with HCC

Journal: World Journal of Surgical Oncology

Article Title: ECM1 promotes migration and invasion of hepatocellular carcinoma by inducing epithelial-mesenchymal transition

doi: 10.1186/s12957-016-0952-z

Figure Lengend Snippet: Multivariate survival analysis of OS and DFS in 120 patients with HCC

Article Snippet: The sections were then immunostained with primary antibody of rabbit anti-ECM1 antibody (11521-1-AP, Proteintech Group, USA) and mouse anti-Vimentin antibody (ZM-0260, ZBGB-BIO) at 4 °C overnight.

Techniques:

a Representative pictures of ECM1 staining in Bel-7402 cells, ECM1 mainly expressed in the cytoplasm of cells. b Representative pictures of ECM1 staining in ECM1-Bel-7402 cells. The ECM1 level of Bel-7402 cells was significantly increased detected by immunohistochemical staining. Original magnification at ×200

Journal: World Journal of Surgical Oncology

Article Title: ECM1 promotes migration and invasion of hepatocellular carcinoma by inducing epithelial-mesenchymal transition

doi: 10.1186/s12957-016-0952-z

Figure Lengend Snippet: a Representative pictures of ECM1 staining in Bel-7402 cells, ECM1 mainly expressed in the cytoplasm of cells. b Representative pictures of ECM1 staining in ECM1-Bel-7402 cells. The ECM1 level of Bel-7402 cells was significantly increased detected by immunohistochemical staining. Original magnification at ×200

Article Snippet: The sections were then immunostained with primary antibody of rabbit anti-ECM1 antibody (11521-1-AP, Proteintech Group, USA) and mouse anti-Vimentin antibody (ZM-0260, ZBGB-BIO) at 4 °C overnight.

Techniques: Staining, Immunohistochemical staining

a Changes of ECM1 and EMT-related protein (E-cadherin and Vimentin) expression in Bel-7402 cells were detected by Western blot after ECM1 transfection. The increased expression of ECM1 could lead to the up-regulated expression of Vimentin protein and down-regulated expression of E-cadherin protein in Bel-7402 cells. b The relative quantity of three protein expressed levels in cells with significant difference (ECM1: t = 12.909, P < 0.01; Vimentin; t = 10.564, P < 0.01; E-cadherin: t = −8.558, P = 0.002). Relative quantity of three protein expressed levels in cells. Data are mean ± SD of three replicates. ( P < 0.05)

Journal: World Journal of Surgical Oncology

Article Title: ECM1 promotes migration and invasion of hepatocellular carcinoma by inducing epithelial-mesenchymal transition

doi: 10.1186/s12957-016-0952-z

Figure Lengend Snippet: a Changes of ECM1 and EMT-related protein (E-cadherin and Vimentin) expression in Bel-7402 cells were detected by Western blot after ECM1 transfection. The increased expression of ECM1 could lead to the up-regulated expression of Vimentin protein and down-regulated expression of E-cadherin protein in Bel-7402 cells. b The relative quantity of three protein expressed levels in cells with significant difference (ECM1: t = 12.909, P < 0.01; Vimentin; t = 10.564, P < 0.01; E-cadherin: t = −8.558, P = 0.002). Relative quantity of three protein expressed levels in cells. Data are mean ± SD of three replicates. ( P < 0.05)

Article Snippet: The sections were then immunostained with primary antibody of rabbit anti-ECM1 antibody (11521-1-AP, Proteintech Group, USA) and mouse anti-Vimentin antibody (ZM-0260, ZBGB-BIO) at 4 °C overnight.

Techniques: Expressing, Western Blot, Transfection

a Effect of ECM1 on the migration capacity of Bel-7402 cell by the wound healing assay at 24, 48, and 72 h (×40). b Statistics of analysis of migration rate date in Bel-7402 and ECM1-Bel-7402 cells. The wound healing assay revealed that the migration rates of the cells in the Bel-7402 group infected with pEGFP-N2-ECM1 were significantly increased compared with those without transfected group ( F = 110.592, P < 0.01). Data are mean ± SD of three replicates. ( P < 0.05)

Journal: World Journal of Surgical Oncology

Article Title: ECM1 promotes migration and invasion of hepatocellular carcinoma by inducing epithelial-mesenchymal transition

doi: 10.1186/s12957-016-0952-z

Figure Lengend Snippet: a Effect of ECM1 on the migration capacity of Bel-7402 cell by the wound healing assay at 24, 48, and 72 h (×40). b Statistics of analysis of migration rate date in Bel-7402 and ECM1-Bel-7402 cells. The wound healing assay revealed that the migration rates of the cells in the Bel-7402 group infected with pEGFP-N2-ECM1 were significantly increased compared with those without transfected group ( F = 110.592, P < 0.01). Data are mean ± SD of three replicates. ( P < 0.05)

Article Snippet: The sections were then immunostained with primary antibody of rabbit anti-ECM1 antibody (11521-1-AP, Proteintech Group, USA) and mouse anti-Vimentin antibody (ZM-0260, ZBGB-BIO) at 4 °C overnight.

Techniques: Migration, Wound Healing Assay, Infection, Transfection

a , b Effect of ECM1 on the invasion capacity of Bel-7402 and ECM1-Bel-7402 cells by invasion assay (×200). c Statistics of analysis of cells per field in Bel-7402 and ECM1-Bel-7402 cells at 48 h. Compared with the control group, after the up-regulated expression of ECM1, the number of invading Bel-7402 cells was significantly increased (89.3 ± 2.5 cells/well vs 28.7 ± 2.5 cells/well) ( t = 29.524, P < 0.01). Data are mean ± SD of three replicates ( P < 0.05)

Journal: World Journal of Surgical Oncology

Article Title: ECM1 promotes migration and invasion of hepatocellular carcinoma by inducing epithelial-mesenchymal transition

doi: 10.1186/s12957-016-0952-z

Figure Lengend Snippet: a , b Effect of ECM1 on the invasion capacity of Bel-7402 and ECM1-Bel-7402 cells by invasion assay (×200). c Statistics of analysis of cells per field in Bel-7402 and ECM1-Bel-7402 cells at 48 h. Compared with the control group, after the up-regulated expression of ECM1, the number of invading Bel-7402 cells was significantly increased (89.3 ± 2.5 cells/well vs 28.7 ± 2.5 cells/well) ( t = 29.524, P < 0.01). Data are mean ± SD of three replicates ( P < 0.05)

Article Snippet: The sections were then immunostained with primary antibody of rabbit anti-ECM1 antibody (11521-1-AP, Proteintech Group, USA) and mouse anti-Vimentin antibody (ZM-0260, ZBGB-BIO) at 4 °C overnight.

Techniques: Invasion Assay, Control, Expressing